ATP hydrolysis and AMP kinase activities of nonstructural protein 2C of human parechovirus 1.

Molecular detection of proteolytic activity of human parechovirus 2A protein by gene expression

  Parechoviruses form one of the nine genera in the picornaviridae family, and include two human pathogens: Human parechovirus type1 and 2 (Hpev1 and Hpev2). The genome of picornaviruses encodes a single polyprotein, which undergoes a cleavage cascade performed by virus encoded proteases to give the final virus proteins. The primary cleavage occurs by 2A protein and this step is critical for vi...

Regulation of lipid metabolism by the AMP-activated protein kinase.

The AMP-activated protein kinase (AMPK) is the central component of a protein kinase cascade which plays an important role in the regulation of many different aspects of lipid metabolism [1,2]. AMPK is activated 50to 100-fold by phosphorylation by an upstream protein kinase, AMPK kinase (AMPKK) [3]. The key regulators of the AMPK cascade currently appear to be S’-AMP and ATP. AMP activates the ...

Regulation of ecto-5´-nucleotidase by docosahexaenoic acid in human endothelial cells.

BACKGROUND/AIMS Modulation of extracellular adenine nucleotide and adenosine concentrations is one potential mechanism by which docosahexaenoic acid (DHA) may exert beneficial effects in critically ill patients. This study assessed DHA effects on extracellular adenine purines. METHODS Experiments used human pulmonary endothelial cells (HPMEC) and umbilical vein endothelial cells (HUVEC) treat...

The Effect of Cigarette Smoking on Human Sperm Creatine Kinase Activity: As An ATP Buffering System in Sperm

Activation of calcium/calmodulin-dependent kinase II following bovine rotavirus enterotoxin NSP4 expression

Objective(s): The rotavirus nonstructural protein 4 (NSP4) is responsible for the increase in cytoplasmic calcium concentration through a phospholipase C-dependent and phospholipase C-independent pathways in infected cells. It is shown that increasing of intracellular calcium concentration in rotavirus infected cells is associated with the activation of some members of protein kinases family su...